Bioinformatic tools & 

Yeast CRISPR CUREs






Bioinformatic Tools :

BUDDY Bioinformatic Utility for Diagnostic Discovery in Yeast. 

Bioinformatic pipeline to model human variants of unknown significance in yeast and test with functional assays.


CODY  CRISPR Oligo Design in Yeast  

sgRNA cloning and repair template generator for introducing missense mutations in the yeast genome using CRISPR/Cas9 (designed for use with pML104 type plasmids).


DROGA Drug Response of Genetic Alleles 

Pipeline to identify human variants within or near drug-binding pockets and assesses their pharmacogenomic impact through CRISPR-based yeast drug resistance assays. 


ALINEy Aminoacid Lookup Interface for Nonsynomous Entries in Yeast

ALINEy aligns and maps manual mutations between human and yeast for visualization.


noVUS Novel Ortholog Validation Using Saccharomyces 

Software for clinicians to submit VUS mutations for yeast experiential analysis.



Yeast Doubling Time (DT) calculator

Calculates doubling times from 96well liquid culture assays.


Yeast Chronological Lifespan (CLS) Analysis  

Software to analyze yeast CLS assays. 






Biochem CURE Protocols: 

https://docs.google.com/document/u/0/d/1_cXalSlOdv-AxyyMFToCoKeYrXuyyI2h6amFuqhIFQI/mobilebasic#heading=h.gs5fotaatew

 Published protocols coming soon